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Publikationen aus dem AK Rinas (2005 – aktuell) / Publications of the Rinas group (2005 – recent)

PUBLIKATIONEN

2018

  • Quaas, B., Burmeister, L., Li, Z., Nimtz, M., Hoffmann, A. and Rinas, U. (2018) Properties of dimeric, disulfide-linked rhBMP-2 recovered from E. coli derived inclusion bodies by mild extraction or chaotropic solubilisation and subsequent refolding. Process Biochem., (https://dx.doi.org/10.1016/j.procbio.2018.02.001)

 

2017

  • Gieseler, G., Pepelanova, I., Stuckenberg, L., Villain, L., Nölle, V., Odenthal, U., Beutel, S., Rinas, U. and Scheper, T. (2017) Purification of bone morphogenetic protein-2 from refolding mixtures using mixed-mode membrane chromatography. Appl. Microbiol. Biotechnol., 101, 123-130. (https://dx.doi.org/10.1007/s00253-016-7784-1)

  • Rinas, U., Garcia-Fruitós, E., Corchero, J.L., Vázquez, E., Seras-Franzoso, J. and Villaverde, A. (2017) Bacterial inclusion bodies: discovering their better half. Trends Biochem. Sci., 42, 726-737. (https://dx.doi.org/10.1016/j.tibs.2017.01.005)

  • Li, Z., Nimtz, M. and Rinas, U. (2017) Global proteome response of Escherichia coli BL21 to production of human basic fibroblast growth factor in complex and defined medium. Eng. Life Sci., 17, 881-891. (https://dx.doi.org/10.1002/elsc.201700036)

 

2016

  • Gieseler, G., Pepelanova, I., Meyer, A., Villain, L., Beutel, S., Rinas, U. and Scheper, T. (2016) Considerations on the flow configuration of membrane chromatography devices for the purification of human basic fibroblast growth factor from crude lysates. Eng. Life Sci., 16, 697-705. (https://dx.doi.org/ 10.1002/elsc.201600006)

  • Marquard, D., Enders, A., Roth, G., Rinas, U., Scheper, T. and Lindner, P. (2016) In situ Microscopy for online monitoring of cell density in Pichia pastoris cultivations. J. Biotechnol., 234. 90-98. (https://dx.doi.org/10.1016/j.jbiotec.2016.07.024)

  • Pesarrodona, M., Fernández, Y., Foradada, L., Sánchez-Chardi, A., Conchillo-Solé, O., Unzueta, U., Xu, Z., Roldán, M., Villegas, S., Ferrer-Miralles, N., Schwartz Jr, S., Rinas, U., Daura, X., Abasolo, I., Vázquez, E. and Villaverde, A. (2016) Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria. Biofabrication, 8, 025001. (https://dx.doi.org/10.1088/1758-5090/8/2/025001)

  • Rueda, F., Virtudes Céspedes, M., Sánchez-Chardi, A., Seras-Franzoso, J., Pesarrodona, M., Ferrer-Miralles, N., Vázquez, E., Rinas, U., Unzueta, U., Mamat, U., Mangues, R., García-Fruitós, E. and Villaverde, A. (2016) Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli. Microb. Cell Fact., 15, 59. (https://dx.doi.org/10.1186/s12934-016-0457-z)

  • Hoepfner, J., Kleinsorge, M., Papp, O., Ackermann, M., Alfken, S., Rinas, U., Solodenko, W., Kirschning, A., Sgodda, M. and Cantz, T. (2016) Bi-phasic modulation of Wnt-signaling supports efficient foregut endoderm formation from human pluripotent stem cells. Cell Biol. Int., Cell Biol. Int., 40, 534-548. (https://dx.doi.org/10.1002/cbin.10590)

 

2015

  • Zahid, M., Lünsdorf, H. and Rinas, U. (2015) Assessing stability and assembly of the hepatitis B surface antigen into virus-like particles during down-stream processing. Vaccine, 33, 3739-3745. (https://dx.doi.org/10.1016/j.vaccine.2015.05.066)

 

2014

  • Li, Z., Carstensen, B. and Rinas, U. (2014) Smart sustainable bottle (SSB) system for E. coli based recombinant protein production. Microb. Cell Fact., 13, 153. (https://dx.doi.org/10.1186/s12934-014-0153-9)

  • Li, Z., Nimtz, M. and Rinas, U. (2014) The metabolic potential of Escherichia coli BL21 in defined and rich medium. Microb. Cell Fact., 13, 45. (https://dx.doi.org/10.1186/1475-2859-13-45)

  • Vanz, A.L., Nimtz, M. and Rinas, U. (2014) Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures. Microb. Cell Fact., 13, 23. (https://dx.doi.org/10.1186/1475-2859-13-23)

  • Ricke-Hoch, M., Bultmann, I., Stapel, B., Condorelli, G., Rinas, U., Sliwa, K., Scherr, M. and Hilfiker-Kleiner, D. (2014) Opposing roles of Akt and STAT3 in protection of the maternal heart from peripartum stress. Cardiovasc. Res., 101, 587-596. (https://dx.doi.org/10.1093/cvr/cvu010)

  • Seras-Franzoso, J., Peebo, K., García-Fruitós, E., Esther Vázquez, E., Rinas, U. and Villaverde, A. (2014) Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates. Acta Biomater., 10, 1354-1359. (https://dx.doi.org/10.1016/j.actbio.2013.12.021)

 

2013

  • Gurramkonda, C., Zahid, M., Nemani, S.K., Adnan, A., Gudi, S.K., Khanna, N., Ebensen, T., Lünsdorf, H., Guzmán, C.A. and Rinas, U. (2013) Purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris and in vivo analysis of their immunogenic properties. J. Chromatogr. B, 940, 104-111. (https://dx.doi.org/10.1016/j.jchromb.2013.09.030)

  • Kaleta, C., Schäuble, S., Rinas, U. and Schuster, S. (2013) Metabolic costs of amino acid and protein production in Escherichia coli. Biotechnol. J., 8, 1105-1114. (https://dx.doi.org/10.1002/biot.201200267)

  • Khetarpal, N., Poddar, A., Nemani, S.K., Dhar, N., Patil, A., Negi, P., Perween, A., Viswanathan, R., Lünsdorf, H., Tyagi, P., Raut, R., Arora, U., Jain, S.K., Rinas, U., Swaminathan, S. and Khanna, N. (2013) Dengue-specific subviral nanoparticles: design, creation and characterization. J. Nanobiotechnol., 11, 15. (https://dx.doi.org/10.1186/1477-3155-11-15)

  • Seras-Franzoso, J., Peebo, K., Corchero, J.L., Tsimbouri, P.M., Unzueta, U., Rinas, U., Dalby, M.J., Vazquez, E., García-Fruitós, E. and Villaverde, A. (2013) A nanostructured bacterial bioscaffold for the sustained bottom-up delivery of protein drugs. Nanomedicine, 8, 1587-1599. (https://dx.doi.org/10.2217/nnm.12.188)

  • Bice, I., Celik, H., Wolff, C., Beutel, S., Zahid, M., Hitzmann, B., Rinas, U., Kasper, C., Gerady-Schahn, R. and Scheper, T. (2013) Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering. Eng. Life Sci., 13, 140-148. (https://dx.doi.org/10.1002/elsc.201200041)

  • Cano-Garrido, O., Rodríguez-Carmona, E., Díez-Gil, C., Vázquez, E., Elizondo, E., Cubarsi, R., Seras-Franzoso, J., Corchero, J.L., Rinas, U., Ratera, I., Ventosa, N., Veciana, J., Villaverde, A. and García-Fruitós, E. (2013) Supramolecular organization of protein-releasing functional amyloids solved in bacterial inclusion bodies. Acta Biomater., 9, 6134-6142. (https://dx.doi.org/10.1016/j.actbio.2012.11.033)

  • Vallejo, L.F. and Rinas, U. (2013) Folding and dimerization kinetics of bone morphogenetic protein-2, a member of the transforming growth factor family. FEBS J., 280, 83-92. (https://dx.doi.org/10.1111/febs.12051)

 

2012

  • Kremling, A., Goehler, A., Jahreis, K., Nees, M., Auerbach, B., Schmidt-Heck, W., Kökpinar, Ö., Geffers, R., Rinas, U. and Bettenbrock, K. (2012) Analysis and design of stimulus response curves in central metabolism of E. coli. Metabolites, 2, 844-871. (https://dx.doi.org/10.3390/metabo2040844)

  • Vanz, A.L., Lünsdorf, H., Adnan, A., Nimtz, M., Gurramkonda, C., Khanna, N. and Rinas, U. (2012) Physiological response of Pichia pastoris 115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes. Microb. Cell Fact., 11, 103. (https://dx.doi.org/10.1186/1475-2859-11-103)

  • Villaverde, A., García-Fruitós, E., Rinas, U., Seras-Franzoso, J., Kosoy, A., Corchero, J.L. and Vazquez, E. (2012) Packaging protein drugs as bacterial inclusion bodies for therapeutic applications. Microb. Cell Fact., 11, 76. (https://dx.doi.org/10.1186/1475-2859-11-76)

  • Vazquez, E., Corchero, J.L., Burgueño, J.F., Seras-Franzoso, J., Kosoy, A., Bosser, R., Mendoza, R., Martínez-Lainez, J.M., Rinas, U., Fernandez, E., Ruiz-Avila, L., García-Fruitós, E. and Villaverde, A. (2012) Functional inclusion bodies produced in bacteria as naturally occurring nanopills for advanced cell therapies. Adv. Mater., 24, 1742-1747. (https://dx.doi.org/10.1002/adma.201104330)

  • Sans, C., García-Fruitós, E., Ferraz, R.M., González-Montalbán, N., Rinas, U., Lopez-Santin, J., Villaverde, A. and Álvaro, G. (2012) Inclusion bodies of fuculose-1-phosphate aldolase as stable and reusable biocatalysts. Biotechnol. Prog., 28, 421-427. (https://dx.doi.org/10.1002/btpr.1518)

  • Chen, R., John, J, Lavrentieva, A., Müller, S., Tomala, M., Zhao, Y., Zweigerdt, R., Beutel, S., Hitzmann, B., Kasper, C., Martin, U., Rinas, U., Stahl, F. and Scheper, T. (2012) Cytokine production using membrane adsorbers: human basic fibroblast growth factor produced by Escherichia coli. Eng. Life Sci., 12, 29-38. (https://dx.doi.org/10.1002/elsc.201100045)

 

2011

  • Göhler, A.-K., Kökpinar, Ö., Schmidt-Heck, W., Geffers, R., Guthke, R., Rinas, U., Schuster, S., Jahreis, J. and Kaleta, C. (2011) More than just a metabolic regulator – elucidation and validation of new targets of PdhR in Escherichia coli. BMC Syst. Biol., 5, 197. (https://dx.doi.org/10.1186/1752-0509-5-197)

  • Li, Z., Nimtz, M. and Rinas, U. (2011) Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems. Appl. Microbiol. Biotechnol., 92, 823-833. (https://dx.doi.org/10.1007/s00253-011-3603-x)

  • Jain, G., Jayaraman, G., Kökpinar, Ö., Rinas, U. and Hitzmann, B. (2011) On-line monitoring of recombinant bacterial cultures using multi-wavelength fluorescence spectroscopy. Biochem. Eng. J., 58-59, 133-139. (https://dx.doi.org/10.1016/j.bej.2011.09.005)

  • Gueorguieva, L., Palani, S., Rinas, U., Jayaraman, G. and Seidel-Morgenstern, A. (2011) Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography - Part II: Process design and experimental validation. J. Chromatogr. A, 1218, 6402-6411. (https://dx.doi.org/10.1016/j.chroma.2011.07.008)

  • Palani, S., Gueorguieva, L., Rinas, U., Seidel-Morgenstern, A. and Jayaraman, G. (2011) Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I: Selection of chromatographic system and estimation of adsorption isotherms. J. Chromatogr. A, 1218, 6396-6401. (https://dx.doi.org/10.1016/j.chroma.2011.06.111)

  • Lünsdorf, H., Gurramkonda, C., Adnan, A., Khanna, N. and Rinas, U. (2011) Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen. Microb. Cell Fact., 10, 48. (https://dx.doi.org/10.1186/1475-2859-10-48)

  • Li, Z., Kessler, W., van den Heuvel, J. and Rinas, U. (2011) Simple defined autoinduction medium for high-level recombinant protein production for T7-based Escherichia coli expression systems. Appl. Microbiol. Biotechnol., 91, 1203-1213. (https://dx.doi.org/10.1007/s00253-011-3407-z)

  • Bals, C., Schambach, A., Meyer, J., Scheper, T. and Rinas, U. (2011) Expression and purification of bioactive soluble murine stem cell factor from recombinant Escherichia coli using thioredoxin as fusion partner. J. Biotechnol., 152, 1-8. (https://dx.doi.org/10.1016/j.jbiotec.2011.01.012)

 

2010

  • Martínez-Alonso, M., García-Fruitós, E., Ferrer-Miralles, N., Rinas, U. and Villaverde, A. (2010) Side effects of chaperone gene co-expression in recombinant protein production. Microb. Cell Fact., 9, 64. (https://dx.doi.org/10.1186/1475-2859-9-64)

  • Gurramkonda, C., Polez, S., Skoko, N., Adnan, A., Gäbel, T., Chugh, D., Swaminathan, S., Khanna, N., Tisminetzky, S. and Rinas, U. (2010) Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin. Microb. Cell Fact., 9, 31. (https://dx.doi.org/10.1186/1475-2859-9-31)

  • Lu, X., Sun, J., Nimtz, M., Wissing, J., Zeng, A.-P. and Rinas, U. (2010) The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate. Microb. Cell Fact., 9, 23. (https://dx.doi.org/10.1186/1475-2859-9-23)

  • Tomala, M., Lavrentieva, A., Moretti, P., Rinas, U., Kasper, C., Stahl, F., Schambach, A., Wahrlich, E., Martin, U., Cantz, T. and Scheper, T. (2010) Preparation of bioactive soluble human leukemia inhibitory factor from recombinant Escherichia coli using thioredoxin as fusion partner. Protein Expr. Purif., 73, 51-57. (https://dx.doi.org/10.1016/j.pep.2010.04.002)

 

2009

  • Gurramkonda, C., Adnan, A., Gäbel, T., Lünsdorf, H., Ross, A., Nemani, S.K., Swaminathan, S., Khanna, N. and Rinas, U. (2009) Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen. Microb. Cell Fact., 8, 13. (https://dx.doi.org/10.1186/1475-2859-8-13)

  • Visser, R., Arrabal, P.M., Becerra, J., Rinas, U. and Cifuentes, M. (2009) The effect of an rhBMP-2 absorbable collagen sponge-targeted system on bone formation in vivo. Biomaterials, 30, 2032-2037. (https://dx.doi.org/10.1016/j.biomaterials.2008.12.046)

 

2008

  • Ganzlin, M. and Rinas, U. (2008) In-depth analysis of the Aspergillus niger glucoamylase (glaA) promoter performance using high-throughput screening and controlled bioreactor cultivation techniques. J. Biotechnol., 135, 266-271. (https://dx.doi.org/10.1016/j.jbiotec.2008.04.005)

  • Gasser, B., Saloheimo, M., Rinas, U., Dragosits, M., Rodríguez-Carmora, E., Baumann, K., Giuliani, M., Parrilli, E., Branduardi, P., Lang, C., Porro, D., Ferrer, P., Tutino, M. L., Mattanovich, D. and Villaverde, A. (2008) Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview. Microb. Cell Fact., 7, 11. (https://dx.doi.org/10.1186/1475-2859-7-11)

 

2007

  • Keßler, L.C., Gueorguieva, L., Rinas, U. and Seidel-Morgenstern, A. (2007) Step gradients in 3-Zone simulated moving bed chromatography: Application to the purification of antibodies and bone morphogenetic protein 2. J. Chromatogr. A, 1176, 69-78. (https://dx.doi.org/10.1016/j.chroma.2007.10.087)

  • Ganzlin, M., Marose, S., Lu, X., Hitzmann, B., Scheper, T., and Rinas, U. (2007) In-situ multi-wavelength fluorescence spectroscopy as effective tool to simultaneously monitor spore germination, metabolic activity and quantitative protein production in recombinant Aspergillus niger fed-batch cultures. J. Biotechnol., 132, 461-468. (https://dx.doi.org/10.1016/j.jbiotec.2007.08.032)

  • Wittmann, C., Weber, J., Betiku, E., Krömer, J., Böhm, D. and Rinas, U. (2007) Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli. J. Biotechnol., 132, 375-384. (https://dx.doi.org/10.1016/j.jbiotec.2007.07.495)

  • Sun, J., Lu, X., Rinas, U. and Zeng, A.-P. (2007) Metabolic peculiarities of Aspergillus niger disclosed by comparative metabolic genomics. Genome Biol., 8, R182. (https://dx.doi.org/10.1186/gb-2007-8-9-r182)

  • Pel, H.J., de Winde, J.H., Archer, D.B., Dyer, P.S., Hofmann, G., Schaap, P.J., Turner, G., de Vries, R.P., Albang, R., Albermann, K., Andersen, M.R., Bendtsen, J.D., Benen, J.A.E., van den Berg, M., Breestraat, S., Caddick, M.X., Contreras, R., Cornell, M., Coutinho, P.M., Danchin, E.G.J., Debets, A.J.M., Dekker, P., van Dijck, P.W.M., van Dijk, A., Dijkhuizen, L., Driessen, A.J.M., D'Enfert, C., Geysens, S., Goosen, C., Groot, G.S.P., de Groot, P.W.J., Guillemette, T., Henrissat, B., Herweijer, M., van den Hombergh, J.P.T.W., van den Hondel, C.A.M.J.J., van der Heijden, R.T.J.M., van der Kaaij, R.M., Klis, F.M., Kools, H.J., Kubicek, C.P., van Kuyk, P.A., Lauber, J., Lu, X., van der Maarel, M.J.E.C., Meulenberg, R., Menke, H., Mortimer, A.M., Nielsen, J., Oliver, S.G., Olsthoorn, M., Pal, K., van Peij, N.N.M.E., Ram, A.F.J., Rinas, U., Roubos, J.A., Sagt, C.M.J., Schmoll, M., Sun, J., Ussery, D., Varga, J., Vervecken, W., van de Vondervoort, P.J.I., Wedler, H., Wösten, H.A.B., Zeng, A.P., van Ooyen, A.J.J., Visser, J. and Stam, H. (2007) Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nature Biotechnol., 25, 221-231. (https://dx.doi.org/10.1038/nbt1282)

  • Rinas, U., Hoffmann, F., Betiku, E., Estapé, D. and Marten, S. (2007) Inclusion body anatomy and functioning of chaperone-mediated in-vivo inclusion body disassembly during high-level recombinant protein production in Escherichia coli. J. Biotechnol., 127, 244-247. (https://dx.doi.org/10.1016/j.jbiotec.2006.07.004)

 

2006

  • Gueorguieva, L., Vallejo, L.F., Rinas, U. and Seidel-Morgenstern, A. (2006) Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches. J. Chromatogr. A, 1135, 142-150. (https://dx.doi.org/10.1016/j.chroma.2006.08.061)

  • El-Enshasy, H., Kleine, J. and Rinas, U. (2006) Agitation effects on morphology and protein productive fractions of filamentous and pelleted growth forms of recombinant Aspergillus niger. Process Biochem., 41, 2103-2112. (https://dx.doi.org/10.1016/j.procbio.2006.05.024)

 

2005

  • Kadow, S., Betiku, E., Rinas, U. and Bilitewski, U. (2005) Development of a rapid, quantitative glucosyltransferase assay based on a screen-printed fructose enzyme electrode and application to optimization studies on gtfD expression in recombinant Escherichia coli. Biotechnol. Bioeng., 91, 154-161. (https://dx.doi.org/10.1002/bit.20501)

  • Rinas, U., El-Enshasy, H., Emmler, M., Hille, A., Hempel, D.C. and Horn, H. (2005) Model-based prediction of substrate conversion and protein synthesis and excretion in recombinant Aspergillus niger biopellets. Chem. Eng. Sci., 60, 2729-2739. (https://dx.doi.org/10.1016/j.ces.2004.12.020)

  • Wendler, J., Vallejo, L.F., Rinas, U. and Bilitewski, U. (2005) Application of an SPR-based receptor assay for the determination of biologically active recombinant bone morphogenetic protein-2. Anal. Bioanal. Chem., 381, 1056-1064. (https://dx.doi.org/10.1007/s00216-004-3016-0)

  • Weber, J., Kayser, A. and Rinas, U. (2005) Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture: II. Dynamic response to famine and feast, activation of the methylglyoxal pathway, and oscillatory behavior. Microbiology, 151, 707-716. (https://dx.doi.org/10.1099/mic.0.27482-0)

  • Kayser, A.,  Weber, J., Hecht, V. and Rinas, U. (2005) Metabolic flux analysis of Escherichia coli in glucose-limited continuous culture: I. Growth rate dependent metabolic efficiency at steady state. Microbiology, 151, 693-706. (https://dx.doi.org/10.1099/mic.0.27481-0)

 

(Lehr-)buchbeiträge

  • Shiloach, J. and Rinas, U. (2010) Bacterial cultivation for production of proteins and other biological products. In Manual of industrial microbiology and biotechnology, 3rd ed. (Baltz, R.H., Davies, J.E. and Demain, A.; Eds.). American Society for Microbiology (ASM), Washington DC, 132-144. (https://dx.doi.org/10.1128/9781555816827.ch10)

  • Shiloach, J. and Rinas, U. (2010) Bacterial cultivation for production of proteins and other biological products. In Manual of industrial microbiology and biotechnology, 3rd ed. (Baltz, R.H., Davies, J.E. and Demain, A.; Eds.). American Society for Microbiology (ASM), Washington DC, 132-144. (https://dx.doi.org/10.1128/9781555816827.ch10)

  • Shiloach, J. and Rinas, U. (2009) Glucose and acetate metabolism in E. coli - System level analysis and biotechnological applications in protein production processes. In Systems biology and biotechnology of Escherichia coli (Lee, S.-Y.; Ed.). Springer-Verlag, Berlin, Heidelberg, New York. p. 377-400. (http://link.springer.com/chapter/10.1007/978-1-4020-9394-4_18#page-1)

  • García-Fruitós, E., González-Montalbán, N., Martínez-Alonso, M., Rinas, U. and Villaverde, A. (2009) Systems-level analysis of protein quality in inclusion body-forming Escherichia coli cells. In Systems biology and biotechnology of Escherichia coli (Lee, S.-Y.; Ed.). Springer-Verlag, Berlin, Heidelberg, New York. p. 295-326. (http://link.springer.com/chapter/10.1007/978-1-4020-9394-4_15#page-2)

  • Rinas, U. (2005) Mikrobielle Herstellung von Pharmaproteinen. In Angewandte Mikrobiologie (Antranikian, G.; Hrsg.) Springer-Verlag, Berlin, Heidelberg, New York. S. 117-133 (http://link.springer.com/chapter/10.1007/3-540-29456-2_6#page-1)